a alternata Search Results


a. alternata extract  (Itea Inc)
90
Itea Inc a. alternata extract
A. Alternata Extract, supplied by Itea Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alternata allergen  (Greer Laboratories)
90
Greer Laboratories alternata allergen
(A) <t>Alternaria</t> <t>alternata</t> and Aspergillus fumigatus -Fungal allergen mix (FAM) was administered intranasally (i.n.) to C57BL/6 (WT) mice every other day (D0, D2, D4) and on D5, the sinonasal wash fluid and respiratory tissue were collected. (B) Levels of interleukin (IL)-5, IL-13, and IL-33 in the sinonasal wash fluid. (C-D) The number of (C) eosinophils and (D) Group 2 Innate Lymphoid Cells (ILC2s) in respiratory tissue as determined by flow cytometry. (E) Top: immunofluorescent staining of respiratory epithelium from PBS or FAM-treated mice (D5) to identify DCLK1+ cells (green with yellow asterisks). Bottom: co-immunostaining for DCLK1 (green) and keratin 5+ cells (white) lining the airway (AW), scale bars = 100μm. (F) Left: flow cytometry gating strategy to identify DCLK1+CD45-Tuft-1 and DCLK1+CD45+ Tuft-2 cells (PBS-treated at top and FAM D5-treated at bottom). Right: total cell numbers recovered from respiratory epithelium of individual mice. (G) Sinonasal wash levels of IL-25 (top) and cysteinyl leukotrienes (CysLT) (bottom). (H) Respiratory epithelium of PBS (left) or FAM-treated mice (D5, right) depicting immunofluorescence staining to identify cells co-expressing keratin 5 (white) and Ki-67+ (magenta) lining the airway (AW) of sinonasal respiratory tissue (scale bar = 100μm). (I) Left: gating strategy used to identify Integrin β4+ Nerve Grow Factor Receptor+ (NGFR) basal cells that express Ki-67 from PBS or FAM D5-treated mice. Right: quantification of cell numbers in respiratory epithelium of individual mice. (J) Model for administration of Dermatophagoides pteronyssinus -House Dust Mite (HDM) extract using the protocol in (A). (K) Levels of IL-5 and IL-13 in sinonasal fluid. (L-N) Total number of (L) eosinophils, (M) ILC2s, (N) Tuft-1 and Tuft-2 cells as determined by flow cytometry. (O) Levels of IL-25 and CysLT in sinonasal fluid. (P) Cell numbers of Ki67+ Integrin β4+ NGFR+ basal cells from the respiratory epithelium of PBS or HDM-treated mice at D5. Data are mean ± SEM, representative of 3-4 experiments (4-5 mice/treatment), T-Test. *** p<0.001, **** p<0.0001. See also .
Alternata Allergen, supplied by Greer Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pathovars of a. alternata  (Rotem Industries)
90
Rotem Industries pathovars of a. alternata
(A) <t>Alternaria</t> <t>alternata</t> and Aspergillus fumigatus -Fungal allergen mix (FAM) was administered intranasally (i.n.) to C57BL/6 (WT) mice every other day (D0, D2, D4) and on D5, the sinonasal wash fluid and respiratory tissue were collected. (B) Levels of interleukin (IL)-5, IL-13, and IL-33 in the sinonasal wash fluid. (C-D) The number of (C) eosinophils and (D) Group 2 Innate Lymphoid Cells (ILC2s) in respiratory tissue as determined by flow cytometry. (E) Top: immunofluorescent staining of respiratory epithelium from PBS or FAM-treated mice (D5) to identify DCLK1+ cells (green with yellow asterisks). Bottom: co-immunostaining for DCLK1 (green) and keratin 5+ cells (white) lining the airway (AW), scale bars = 100μm. (F) Left: flow cytometry gating strategy to identify DCLK1+CD45-Tuft-1 and DCLK1+CD45+ Tuft-2 cells (PBS-treated at top and FAM D5-treated at bottom). Right: total cell numbers recovered from respiratory epithelium of individual mice. (G) Sinonasal wash levels of IL-25 (top) and cysteinyl leukotrienes (CysLT) (bottom). (H) Respiratory epithelium of PBS (left) or FAM-treated mice (D5, right) depicting immunofluorescence staining to identify cells co-expressing keratin 5 (white) and Ki-67+ (magenta) lining the airway (AW) of sinonasal respiratory tissue (scale bar = 100μm). (I) Left: gating strategy used to identify Integrin β4+ Nerve Grow Factor Receptor+ (NGFR) basal cells that express Ki-67 from PBS or FAM D5-treated mice. Right: quantification of cell numbers in respiratory epithelium of individual mice. (J) Model for administration of Dermatophagoides pteronyssinus -House Dust Mite (HDM) extract using the protocol in (A). (K) Levels of IL-5 and IL-13 in sinonasal fluid. (L-N) Total number of (L) eosinophils, (M) ILC2s, (N) Tuft-1 and Tuft-2 cells as determined by flow cytometry. (O) Levels of IL-25 and CysLT in sinonasal fluid. (P) Cell numbers of Ki67+ Integrin β4+ NGFR+ basal cells from the respiratory epithelium of PBS or HDM-treated mice at D5. Data are mean ± SEM, representative of 3-4 experiments (4-5 mice/treatment), T-Test. *** p<0.001, **** p<0.0001. See also .
Pathovars Of A. Alternata, supplied by Rotem Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a. alternata spores  (Fisher Scientific)
90
Fisher Scientific a. alternata spores
(A) <t>Alternaria</t> <t>alternata</t> and Aspergillus fumigatus -Fungal allergen mix (FAM) was administered intranasally (i.n.) to C57BL/6 (WT) mice every other day (D0, D2, D4) and on D5, the sinonasal wash fluid and respiratory tissue were collected. (B) Levels of interleukin (IL)-5, IL-13, and IL-33 in the sinonasal wash fluid. (C-D) The number of (C) eosinophils and (D) Group 2 Innate Lymphoid Cells (ILC2s) in respiratory tissue as determined by flow cytometry. (E) Top: immunofluorescent staining of respiratory epithelium from PBS or FAM-treated mice (D5) to identify DCLK1+ cells (green with yellow asterisks). Bottom: co-immunostaining for DCLK1 (green) and keratin 5+ cells (white) lining the airway (AW), scale bars = 100μm. (F) Left: flow cytometry gating strategy to identify DCLK1+CD45-Tuft-1 and DCLK1+CD45+ Tuft-2 cells (PBS-treated at top and FAM D5-treated at bottom). Right: total cell numbers recovered from respiratory epithelium of individual mice. (G) Sinonasal wash levels of IL-25 (top) and cysteinyl leukotrienes (CysLT) (bottom). (H) Respiratory epithelium of PBS (left) or FAM-treated mice (D5, right) depicting immunofluorescence staining to identify cells co-expressing keratin 5 (white) and Ki-67+ (magenta) lining the airway (AW) of sinonasal respiratory tissue (scale bar = 100μm). (I) Left: gating strategy used to identify Integrin β4+ Nerve Grow Factor Receptor+ (NGFR) basal cells that express Ki-67 from PBS or FAM D5-treated mice. Right: quantification of cell numbers in respiratory epithelium of individual mice. (J) Model for administration of Dermatophagoides pteronyssinus -House Dust Mite (HDM) extract using the protocol in (A). (K) Levels of IL-5 and IL-13 in sinonasal fluid. (L-N) Total number of (L) eosinophils, (M) ILC2s, (N) Tuft-1 and Tuft-2 cells as determined by flow cytometry. (O) Levels of IL-25 and CysLT in sinonasal fluid. (P) Cell numbers of Ki67+ Integrin β4+ NGFR+ basal cells from the respiratory epithelium of PBS or HDM-treated mice at D5. Data are mean ± SEM, representative of 3-4 experiments (4-5 mice/treatment), T-Test. *** p<0.001, **** p<0.0001. See also .
A. Alternata Spores, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a. alternata spores - by Bioz Stars, 2026-03
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100 µg a. alternata (greerlabs) in 40 µl pbs for four consecutive days  (Greer Laboratories)
90
Greer Laboratories 100 µg a. alternata (greerlabs) in 40 µl pbs for four consecutive days
ICAM-1 deficiency impairs ILC2 function in response to IL-33 challenge. (A and B) BM cells from WT mice were cultured in the presence of IL-7 (10 ng/ml) with <t>PBS</t> or IL-33 (10 ng/ml) treatment. 2 d later, the expression of ICAM-1 (A) or LFA-1 (B) on BM iILC2s was evaluated by flow cytometry. (C) Human PBMCs were cultured in the presence of recombinant human IL-2 (20 ng/ml) and IL-7 (20 ng/ml) with PBS or human IL-33 (20 ng/ml) treatment for 3 d. The MFI levels of ICAM-1 and LFA-1 on human ILC2s (CD45 + Lin − CD127 + CRTH2 + ) were evaluated by flow cytometry. (D) Equal number of purified lung ILC2s from WT and ICAM-1 −/− mice were cultured in the presence of IL-2 (10 ng/ml), IL-7 (20 ng/ml), and IL-33 (20 ng/ml) for 3 d, and the amount of IL-5 and IL-13 in the supernatants was evaluated by ELISA. (E–I) WT and ICAM-1 −/− mice <t>were</t> <t>intranasally</t> administered with IL-33 (500 ng/mouse/d) or PBS for three consecutive days ( n = 6 for each group). Mice were sacrificed on day 4. (E) Representative flow cytometric analysis of ILC2, pregated on CD45 + Lin − , and the number of ILC2 in lung from WT and ICAM-1 −/− mice after PBS or IL-33 treatment. (F) Frequencies of IL-5 + IL-13 + in lung ILC2s after cell stimulation cocktail treatment for 4 h. IL-33-challenged groups were analyzed. (G) The amount of IL-5 and IL-13 in BAL was determined by ELISA. (H) The number of eosinophils (CD45 + CD11c − Siglec-F + ) in BAL was shown. (I) Representative H&E staining of lung sections (bars, 100 µm). Data are representative of two (E–I) to three (A–D) independent experiments. Error bars show mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001 by unpaired Student's t test. ns, not significant. Numbers within flow plots indicate the percentages of cells gated.
100 µg A. Alternata (Greerlabs) In 40 µl Pbs For Four Consecutive Days, supplied by Greer Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
100 µg a. alternata (greerlabs) in 40 µl pbs for four consecutive days - by Bioz Stars, 2026-03
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recombinant allergens from alternaria alternata fungus alt a 1  (Biomay Inc)
90
Biomay Inc recombinant allergens from alternaria alternata fungus alt a 1
ICAM-1 deficiency impairs ILC2 function in response to IL-33 challenge. (A and B) BM cells from WT mice were cultured in the presence of IL-7 (10 ng/ml) with <t>PBS</t> or IL-33 (10 ng/ml) treatment. 2 d later, the expression of ICAM-1 (A) or LFA-1 (B) on BM iILC2s was evaluated by flow cytometry. (C) Human PBMCs were cultured in the presence of recombinant human IL-2 (20 ng/ml) and IL-7 (20 ng/ml) with PBS or human IL-33 (20 ng/ml) treatment for 3 d. The MFI levels of ICAM-1 and LFA-1 on human ILC2s (CD45 + Lin − CD127 + CRTH2 + ) were evaluated by flow cytometry. (D) Equal number of purified lung ILC2s from WT and ICAM-1 −/− mice were cultured in the presence of IL-2 (10 ng/ml), IL-7 (20 ng/ml), and IL-33 (20 ng/ml) for 3 d, and the amount of IL-5 and IL-13 in the supernatants was evaluated by ELISA. (E–I) WT and ICAM-1 −/− mice <t>were</t> <t>intranasally</t> administered with IL-33 (500 ng/mouse/d) or PBS for three consecutive days ( n = 6 for each group). Mice were sacrificed on day 4. (E) Representative flow cytometric analysis of ILC2, pregated on CD45 + Lin − , and the number of ILC2 in lung from WT and ICAM-1 −/− mice after PBS or IL-33 treatment. (F) Frequencies of IL-5 + IL-13 + in lung ILC2s after cell stimulation cocktail treatment for 4 h. IL-33-challenged groups were analyzed. (G) The amount of IL-5 and IL-13 in BAL was determined by ELISA. (H) The number of eosinophils (CD45 + CD11c − Siglec-F + ) in BAL was shown. (I) Representative H&E staining of lung sections (bars, 100 µm). Data are representative of two (E–I) to three (A–D) independent experiments. Error bars show mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001 by unpaired Student's t test. ns, not significant. Numbers within flow plots indicate the percentages of cells gated.
Recombinant Allergens From Alternaria Alternata Fungus Alt A 1, supplied by Biomay Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alternata extract greer  (Greer Laboratories)
90
Greer Laboratories alternata extract greer
ICAM-1 deficiency impairs ILC2 function in response to IL-33 challenge. (A and B) BM cells from WT mice were cultured in the presence of IL-7 (10 ng/ml) with <t>PBS</t> or IL-33 (10 ng/ml) treatment. 2 d later, the expression of ICAM-1 (A) or LFA-1 (B) on BM iILC2s was evaluated by flow cytometry. (C) Human PBMCs were cultured in the presence of recombinant human IL-2 (20 ng/ml) and IL-7 (20 ng/ml) with PBS or human IL-33 (20 ng/ml) treatment for 3 d. The MFI levels of ICAM-1 and LFA-1 on human ILC2s (CD45 + Lin − CD127 + CRTH2 + ) were evaluated by flow cytometry. (D) Equal number of purified lung ILC2s from WT and ICAM-1 −/− mice were cultured in the presence of IL-2 (10 ng/ml), IL-7 (20 ng/ml), and IL-33 (20 ng/ml) for 3 d, and the amount of IL-5 and IL-13 in the supernatants was evaluated by ELISA. (E–I) WT and ICAM-1 −/− mice <t>were</t> <t>intranasally</t> administered with IL-33 (500 ng/mouse/d) or PBS for three consecutive days ( n = 6 for each group). Mice were sacrificed on day 4. (E) Representative flow cytometric analysis of ILC2, pregated on CD45 + Lin − , and the number of ILC2 in lung from WT and ICAM-1 −/− mice after PBS or IL-33 treatment. (F) Frequencies of IL-5 + IL-13 + in lung ILC2s after cell stimulation cocktail treatment for 4 h. IL-33-challenged groups were analyzed. (G) The amount of IL-5 and IL-13 in BAL was determined by ELISA. (H) The number of eosinophils (CD45 + CD11c − Siglec-F + ) in BAL was shown. (I) Representative H&E staining of lung sections (bars, 100 µm). Data are representative of two (E–I) to three (A–D) independent experiments. Error bars show mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001 by unpaired Student's t test. ns, not significant. Numbers within flow plots indicate the percentages of cells gated.
Alternata Extract Greer, supplied by Greer Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a. alternata  (Rotem Industries)
90
Rotem Industries a. alternata
ICAM-1 deficiency impairs ILC2 function in response to IL-33 challenge. (A and B) BM cells from WT mice were cultured in the presence of IL-7 (10 ng/ml) with <t>PBS</t> or IL-33 (10 ng/ml) treatment. 2 d later, the expression of ICAM-1 (A) or LFA-1 (B) on BM iILC2s was evaluated by flow cytometry. (C) Human PBMCs were cultured in the presence of recombinant human IL-2 (20 ng/ml) and IL-7 (20 ng/ml) with PBS or human IL-33 (20 ng/ml) treatment for 3 d. The MFI levels of ICAM-1 and LFA-1 on human ILC2s (CD45 + Lin − CD127 + CRTH2 + ) were evaluated by flow cytometry. (D) Equal number of purified lung ILC2s from WT and ICAM-1 −/− mice were cultured in the presence of IL-2 (10 ng/ml), IL-7 (20 ng/ml), and IL-33 (20 ng/ml) for 3 d, and the amount of IL-5 and IL-13 in the supernatants was evaluated by ELISA. (E–I) WT and ICAM-1 −/− mice <t>were</t> <t>intranasally</t> administered with IL-33 (500 ng/mouse/d) or PBS for three consecutive days ( n = 6 for each group). Mice were sacrificed on day 4. (E) Representative flow cytometric analysis of ILC2, pregated on CD45 + Lin − , and the number of ILC2 in lung from WT and ICAM-1 −/− mice after PBS or IL-33 treatment. (F) Frequencies of IL-5 + IL-13 + in lung ILC2s after cell stimulation cocktail treatment for 4 h. IL-33-challenged groups were analyzed. (G) The amount of IL-5 and IL-13 in BAL was determined by ELISA. (H) The number of eosinophils (CD45 + CD11c − Siglec-F + ) in BAL was shown. (I) Representative H&E staining of lung sections (bars, 100 µm). Data are representative of two (E–I) to three (A–D) independent experiments. Error bars show mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001 by unpaired Student's t test. ns, not significant. Numbers within flow plots indicate the percentages of cells gated.
A. Alternata, supplied by Rotem Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
a. alternata - by Bioz Stars, 2026-03
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aqueous extracts from a. alternata  (Greer Laboratories)
90
Greer Laboratories aqueous extracts from a. alternata
ICAM-1 deficiency impairs ILC2 function in response to IL-33 challenge. (A and B) BM cells from WT mice were cultured in the presence of IL-7 (10 ng/ml) with <t>PBS</t> or IL-33 (10 ng/ml) treatment. 2 d later, the expression of ICAM-1 (A) or LFA-1 (B) on BM iILC2s was evaluated by flow cytometry. (C) Human PBMCs were cultured in the presence of recombinant human IL-2 (20 ng/ml) and IL-7 (20 ng/ml) with PBS or human IL-33 (20 ng/ml) treatment for 3 d. The MFI levels of ICAM-1 and LFA-1 on human ILC2s (CD45 + Lin − CD127 + CRTH2 + ) were evaluated by flow cytometry. (D) Equal number of purified lung ILC2s from WT and ICAM-1 −/− mice were cultured in the presence of IL-2 (10 ng/ml), IL-7 (20 ng/ml), and IL-33 (20 ng/ml) for 3 d, and the amount of IL-5 and IL-13 in the supernatants was evaluated by ELISA. (E–I) WT and ICAM-1 −/− mice <t>were</t> <t>intranasally</t> administered with IL-33 (500 ng/mouse/d) or PBS for three consecutive days ( n = 6 for each group). Mice were sacrificed on day 4. (E) Representative flow cytometric analysis of ILC2, pregated on CD45 + Lin − , and the number of ILC2 in lung from WT and ICAM-1 −/− mice after PBS or IL-33 treatment. (F) Frequencies of IL-5 + IL-13 + in lung ILC2s after cell stimulation cocktail treatment for 4 h. IL-33-challenged groups were analyzed. (G) The amount of IL-5 and IL-13 in BAL was determined by ELISA. (H) The number of eosinophils (CD45 + CD11c − Siglec-F + ) in BAL was shown. (I) Representative H&E staining of lung sections (bars, 100 µm). Data are representative of two (E–I) to three (A–D) independent experiments. Error bars show mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001 by unpaired Student's t test. ns, not significant. Numbers within flow plots indicate the percentages of cells gated.
Aqueous Extracts From A. Alternata, supplied by Greer Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ethyl acetate extract of endophytic fungus a. alternata vn3  (Vitex Inc)
90
Vitex Inc ethyl acetate extract of endophytic fungus a. alternata vn3
ICAM-1 deficiency impairs ILC2 function in response to IL-33 challenge. (A and B) BM cells from WT mice were cultured in the presence of IL-7 (10 ng/ml) with <t>PBS</t> or IL-33 (10 ng/ml) treatment. 2 d later, the expression of ICAM-1 (A) or LFA-1 (B) on BM iILC2s was evaluated by flow cytometry. (C) Human PBMCs were cultured in the presence of recombinant human IL-2 (20 ng/ml) and IL-7 (20 ng/ml) with PBS or human IL-33 (20 ng/ml) treatment for 3 d. The MFI levels of ICAM-1 and LFA-1 on human ILC2s (CD45 + Lin − CD127 + CRTH2 + ) were evaluated by flow cytometry. (D) Equal number of purified lung ILC2s from WT and ICAM-1 −/− mice were cultured in the presence of IL-2 (10 ng/ml), IL-7 (20 ng/ml), and IL-33 (20 ng/ml) for 3 d, and the amount of IL-5 and IL-13 in the supernatants was evaluated by ELISA. (E–I) WT and ICAM-1 −/− mice <t>were</t> <t>intranasally</t> administered with IL-33 (500 ng/mouse/d) or PBS for three consecutive days ( n = 6 for each group). Mice were sacrificed on day 4. (E) Representative flow cytometric analysis of ILC2, pregated on CD45 + Lin − , and the number of ILC2 in lung from WT and ICAM-1 −/− mice after PBS or IL-33 treatment. (F) Frequencies of IL-5 + IL-13 + in lung ILC2s after cell stimulation cocktail treatment for 4 h. IL-33-challenged groups were analyzed. (G) The amount of IL-5 and IL-13 in BAL was determined by ELISA. (H) The number of eosinophils (CD45 + CD11c − Siglec-F + ) in BAL was shown. (I) Representative H&E staining of lung sections (bars, 100 µm). Data are representative of two (E–I) to three (A–D) independent experiments. Error bars show mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001 by unpaired Student's t test. ns, not significant. Numbers within flow plots indicate the percentages of cells gated.
Ethyl Acetate Extract Of Endophytic Fungus A. Alternata Vn3, supplied by Vitex Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a. alternata  (Kaltenbach GmbH)
90
Kaltenbach GmbH a. alternata
ICAM-1 deficiency impairs ILC2 function in response to IL-33 challenge. (A and B) BM cells from WT mice were cultured in the presence of IL-7 (10 ng/ml) with <t>PBS</t> or IL-33 (10 ng/ml) treatment. 2 d later, the expression of ICAM-1 (A) or LFA-1 (B) on BM iILC2s was evaluated by flow cytometry. (C) Human PBMCs were cultured in the presence of recombinant human IL-2 (20 ng/ml) and IL-7 (20 ng/ml) with PBS or human IL-33 (20 ng/ml) treatment for 3 d. The MFI levels of ICAM-1 and LFA-1 on human ILC2s (CD45 + Lin − CD127 + CRTH2 + ) were evaluated by flow cytometry. (D) Equal number of purified lung ILC2s from WT and ICAM-1 −/− mice were cultured in the presence of IL-2 (10 ng/ml), IL-7 (20 ng/ml), and IL-33 (20 ng/ml) for 3 d, and the amount of IL-5 and IL-13 in the supernatants was evaluated by ELISA. (E–I) WT and ICAM-1 −/− mice <t>were</t> <t>intranasally</t> administered with IL-33 (500 ng/mouse/d) or PBS for three consecutive days ( n = 6 for each group). Mice were sacrificed on day 4. (E) Representative flow cytometric analysis of ILC2, pregated on CD45 + Lin − , and the number of ILC2 in lung from WT and ICAM-1 −/− mice after PBS or IL-33 treatment. (F) Frequencies of IL-5 + IL-13 + in lung ILC2s after cell stimulation cocktail treatment for 4 h. IL-33-challenged groups were analyzed. (G) The amount of IL-5 and IL-13 in BAL was determined by ELISA. (H) The number of eosinophils (CD45 + CD11c − Siglec-F + ) in BAL was shown. (I) Representative H&E staining of lung sections (bars, 100 µm). Data are representative of two (E–I) to three (A–D) independent experiments. Error bars show mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001 by unpaired Student's t test. ns, not significant. Numbers within flow plots indicate the percentages of cells gated.
A. Alternata, supplied by Kaltenbach GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a. alternata mbl-t  (MBL Life science)
90
MBL Life science a. alternata mbl-t
Comparison of glucoamylase production and specific activity by using various raw media for growth of A. <t>alternata.</t>
A. Alternata Mbl T, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Alternaria alternata and Aspergillus fumigatus -Fungal allergen mix (FAM) was administered intranasally (i.n.) to C57BL/6 (WT) mice every other day (D0, D2, D4) and on D5, the sinonasal wash fluid and respiratory tissue were collected. (B) Levels of interleukin (IL)-5, IL-13, and IL-33 in the sinonasal wash fluid. (C-D) The number of (C) eosinophils and (D) Group 2 Innate Lymphoid Cells (ILC2s) in respiratory tissue as determined by flow cytometry. (E) Top: immunofluorescent staining of respiratory epithelium from PBS or FAM-treated mice (D5) to identify DCLK1+ cells (green with yellow asterisks). Bottom: co-immunostaining for DCLK1 (green) and keratin 5+ cells (white) lining the airway (AW), scale bars = 100μm. (F) Left: flow cytometry gating strategy to identify DCLK1+CD45-Tuft-1 and DCLK1+CD45+ Tuft-2 cells (PBS-treated at top and FAM D5-treated at bottom). Right: total cell numbers recovered from respiratory epithelium of individual mice. (G) Sinonasal wash levels of IL-25 (top) and cysteinyl leukotrienes (CysLT) (bottom). (H) Respiratory epithelium of PBS (left) or FAM-treated mice (D5, right) depicting immunofluorescence staining to identify cells co-expressing keratin 5 (white) and Ki-67+ (magenta) lining the airway (AW) of sinonasal respiratory tissue (scale bar = 100μm). (I) Left: gating strategy used to identify Integrin β4+ Nerve Grow Factor Receptor+ (NGFR) basal cells that express Ki-67 from PBS or FAM D5-treated mice. Right: quantification of cell numbers in respiratory epithelium of individual mice. (J) Model for administration of Dermatophagoides pteronyssinus -House Dust Mite (HDM) extract using the protocol in (A). (K) Levels of IL-5 and IL-13 in sinonasal fluid. (L-N) Total number of (L) eosinophils, (M) ILC2s, (N) Tuft-1 and Tuft-2 cells as determined by flow cytometry. (O) Levels of IL-25 and CysLT in sinonasal fluid. (P) Cell numbers of Ki67+ Integrin β4+ NGFR+ basal cells from the respiratory epithelium of PBS or HDM-treated mice at D5. Data are mean ± SEM, representative of 3-4 experiments (4-5 mice/treatment), T-Test. *** p<0.001, **** p<0.0001. See also .

Journal: bioRxiv

Article Title: Neuron-dependent tuft cell expansion initiates sinonasal allergic Type 2 inflammation

doi: 10.1101/2023.07.04.547596

Figure Lengend Snippet: (A) Alternaria alternata and Aspergillus fumigatus -Fungal allergen mix (FAM) was administered intranasally (i.n.) to C57BL/6 (WT) mice every other day (D0, D2, D4) and on D5, the sinonasal wash fluid and respiratory tissue were collected. (B) Levels of interleukin (IL)-5, IL-13, and IL-33 in the sinonasal wash fluid. (C-D) The number of (C) eosinophils and (D) Group 2 Innate Lymphoid Cells (ILC2s) in respiratory tissue as determined by flow cytometry. (E) Top: immunofluorescent staining of respiratory epithelium from PBS or FAM-treated mice (D5) to identify DCLK1+ cells (green with yellow asterisks). Bottom: co-immunostaining for DCLK1 (green) and keratin 5+ cells (white) lining the airway (AW), scale bars = 100μm. (F) Left: flow cytometry gating strategy to identify DCLK1+CD45-Tuft-1 and DCLK1+CD45+ Tuft-2 cells (PBS-treated at top and FAM D5-treated at bottom). Right: total cell numbers recovered from respiratory epithelium of individual mice. (G) Sinonasal wash levels of IL-25 (top) and cysteinyl leukotrienes (CysLT) (bottom). (H) Respiratory epithelium of PBS (left) or FAM-treated mice (D5, right) depicting immunofluorescence staining to identify cells co-expressing keratin 5 (white) and Ki-67+ (magenta) lining the airway (AW) of sinonasal respiratory tissue (scale bar = 100μm). (I) Left: gating strategy used to identify Integrin β4+ Nerve Grow Factor Receptor+ (NGFR) basal cells that express Ki-67 from PBS or FAM D5-treated mice. Right: quantification of cell numbers in respiratory epithelium of individual mice. (J) Model for administration of Dermatophagoides pteronyssinus -House Dust Mite (HDM) extract using the protocol in (A). (K) Levels of IL-5 and IL-13 in sinonasal fluid. (L-N) Total number of (L) eosinophils, (M) ILC2s, (N) Tuft-1 and Tuft-2 cells as determined by flow cytometry. (O) Levels of IL-25 and CysLT in sinonasal fluid. (P) Cell numbers of Ki67+ Integrin β4+ NGFR+ basal cells from the respiratory epithelium of PBS or HDM-treated mice at D5. Data are mean ± SEM, representative of 3-4 experiments (4-5 mice/treatment), T-Test. *** p<0.001, **** p<0.0001. See also .

Article Snippet: To induce sinonasal allergic Type 2 inflammation, mice were anesthetized with isoflurane (1-4%) and treated intranasally (i.n.) with a fungal allergen mix (FAM) consisting of A. alternata allergen (Greer Laboratories, Cat# NC1620293) and A. aspergillus allergen (Greer Laboratories, Cat# NC1677927) 3 times a week for 3 weeks ( ).

Techniques: Flow Cytometry, Staining, Immunostaining, Immunofluorescence, Expressing

(A) PBS or Alternaria alternata and Aspergillus fumigatus -Fungal allergen mix (FAM) was administered intranasally (i.n.) every other day to Pou2f3 -/- mice, lacking mature tuft cells, or their WT littermate controls. Respiratory tissue and sinonasal wash were harvested and analyzed on D5. (B) Immunofluorescence staining of respiratory epithelium from FAM-treated WT or Pou2f3-/- mice to identify DCLK1+ cells (green with yellow asterisks) lining the airway (AW), scale bar = 100 μm. (C) Tuft 1 (left) and tuft 2 (right) cell numbers in the respiratory epithelium as determined by flow cytometry. PBS-treated WT (white), FAM D5-treated WT (Blue), PBS-treated Pou2f -/- (gray), and FAM D5-treated Pou2f -/- (red). (D) Levels of IL-25 (left) and cysteinyl leukotrienes (CysLT, right). (E) Number of total basal cell population (left) and Ki67+ basal cell population (right). (F) Sinonasal fluid levels of IL-5 and IL-13. (G-I) Total numbers of (G) ILC2, (H) CD4+ TH2 cells, and (I) eosinophils in respiratory tissue. Data are mean ± SEM, representative of 1 experiment (4 mice/treatment), one-way ANOVA with Tukey’s post-hoc tests. * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001. See also and .

Journal: bioRxiv

Article Title: Neuron-dependent tuft cell expansion initiates sinonasal allergic Type 2 inflammation

doi: 10.1101/2023.07.04.547596

Figure Lengend Snippet: (A) PBS or Alternaria alternata and Aspergillus fumigatus -Fungal allergen mix (FAM) was administered intranasally (i.n.) every other day to Pou2f3 -/- mice, lacking mature tuft cells, or their WT littermate controls. Respiratory tissue and sinonasal wash were harvested and analyzed on D5. (B) Immunofluorescence staining of respiratory epithelium from FAM-treated WT or Pou2f3-/- mice to identify DCLK1+ cells (green with yellow asterisks) lining the airway (AW), scale bar = 100 μm. (C) Tuft 1 (left) and tuft 2 (right) cell numbers in the respiratory epithelium as determined by flow cytometry. PBS-treated WT (white), FAM D5-treated WT (Blue), PBS-treated Pou2f -/- (gray), and FAM D5-treated Pou2f -/- (red). (D) Levels of IL-25 (left) and cysteinyl leukotrienes (CysLT, right). (E) Number of total basal cell population (left) and Ki67+ basal cell population (right). (F) Sinonasal fluid levels of IL-5 and IL-13. (G-I) Total numbers of (G) ILC2, (H) CD4+ TH2 cells, and (I) eosinophils in respiratory tissue. Data are mean ± SEM, representative of 1 experiment (4 mice/treatment), one-way ANOVA with Tukey’s post-hoc tests. * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001. See also and .

Article Snippet: To induce sinonasal allergic Type 2 inflammation, mice were anesthetized with isoflurane (1-4%) and treated intranasally (i.n.) with a fungal allergen mix (FAM) consisting of A. alternata allergen (Greer Laboratories, Cat# NC1620293) and A. aspergillus allergen (Greer Laboratories, Cat# NC1677927) 3 times a week for 3 weeks ( ).

Techniques: Immunofluorescence, Staining, Flow Cytometry

ICAM-1 deficiency impairs ILC2 function in response to IL-33 challenge. (A and B) BM cells from WT mice were cultured in the presence of IL-7 (10 ng/ml) with PBS or IL-33 (10 ng/ml) treatment. 2 d later, the expression of ICAM-1 (A) or LFA-1 (B) on BM iILC2s was evaluated by flow cytometry. (C) Human PBMCs were cultured in the presence of recombinant human IL-2 (20 ng/ml) and IL-7 (20 ng/ml) with PBS or human IL-33 (20 ng/ml) treatment for 3 d. The MFI levels of ICAM-1 and LFA-1 on human ILC2s (CD45 + Lin − CD127 + CRTH2 + ) were evaluated by flow cytometry. (D) Equal number of purified lung ILC2s from WT and ICAM-1 −/− mice were cultured in the presence of IL-2 (10 ng/ml), IL-7 (20 ng/ml), and IL-33 (20 ng/ml) for 3 d, and the amount of IL-5 and IL-13 in the supernatants was evaluated by ELISA. (E–I) WT and ICAM-1 −/− mice were intranasally administered with IL-33 (500 ng/mouse/d) or PBS for three consecutive days ( n = 6 for each group). Mice were sacrificed on day 4. (E) Representative flow cytometric analysis of ILC2, pregated on CD45 + Lin − , and the number of ILC2 in lung from WT and ICAM-1 −/− mice after PBS or IL-33 treatment. (F) Frequencies of IL-5 + IL-13 + in lung ILC2s after cell stimulation cocktail treatment for 4 h. IL-33-challenged groups were analyzed. (G) The amount of IL-5 and IL-13 in BAL was determined by ELISA. (H) The number of eosinophils (CD45 + CD11c − Siglec-F + ) in BAL was shown. (I) Representative H&E staining of lung sections (bars, 100 µm). Data are representative of two (E–I) to three (A–D) independent experiments. Error bars show mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001 by unpaired Student's t test. ns, not significant. Numbers within flow plots indicate the percentages of cells gated.

Journal: The Journal of Experimental Medicine

Article Title: ICAM-1 controls development and function of ILC2

doi: 10.1084/jem.20172359

Figure Lengend Snippet: ICAM-1 deficiency impairs ILC2 function in response to IL-33 challenge. (A and B) BM cells from WT mice were cultured in the presence of IL-7 (10 ng/ml) with PBS or IL-33 (10 ng/ml) treatment. 2 d later, the expression of ICAM-1 (A) or LFA-1 (B) on BM iILC2s was evaluated by flow cytometry. (C) Human PBMCs were cultured in the presence of recombinant human IL-2 (20 ng/ml) and IL-7 (20 ng/ml) with PBS or human IL-33 (20 ng/ml) treatment for 3 d. The MFI levels of ICAM-1 and LFA-1 on human ILC2s (CD45 + Lin − CD127 + CRTH2 + ) were evaluated by flow cytometry. (D) Equal number of purified lung ILC2s from WT and ICAM-1 −/− mice were cultured in the presence of IL-2 (10 ng/ml), IL-7 (20 ng/ml), and IL-33 (20 ng/ml) for 3 d, and the amount of IL-5 and IL-13 in the supernatants was evaluated by ELISA. (E–I) WT and ICAM-1 −/− mice were intranasally administered with IL-33 (500 ng/mouse/d) or PBS for three consecutive days ( n = 6 for each group). Mice were sacrificed on day 4. (E) Representative flow cytometric analysis of ILC2, pregated on CD45 + Lin − , and the number of ILC2 in lung from WT and ICAM-1 −/− mice after PBS or IL-33 treatment. (F) Frequencies of IL-5 + IL-13 + in lung ILC2s after cell stimulation cocktail treatment for 4 h. IL-33-challenged groups were analyzed. (G) The amount of IL-5 and IL-13 in BAL was determined by ELISA. (H) The number of eosinophils (CD45 + CD11c − Siglec-F + ) in BAL was shown. (I) Representative H&E staining of lung sections (bars, 100 µm). Data are representative of two (E–I) to three (A–D) independent experiments. Error bars show mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001 by unpaired Student's t test. ns, not significant. Numbers within flow plots indicate the percentages of cells gated.

Article Snippet: For A. alternata –induced lung inflammation, mice were intranasally administrated with 100 µg A. alternata (Greerlabs) in 40 µl PBS for four consecutive days as previously described ( ).

Techniques: Cell Culture, Expressing, Flow Cytometry, Recombinant, Purification, Enzyme-linked Immunosorbent Assay, Cell Stimulation, Staining

ICAM-1 deficiency attenuates papain and A. alternata –induced lung inflammation. (A–E) WT and ICAM-1 −/− mice were intranasally administered with papain or PBS for 5 d ( n = 6 for each group). Mice were sacrificed 24 h after the last treatment. (A) The frequencies and number of eosinophils in BAL were evaluated by flow cytometry. (B) The amount of IL-5 and IL-13 in BAL was measured by ELISA. (C) Representative lung histology by H&E staining (bars, 100 µm). (D) The frequencies and number of lung ILC2s were determined by flow cytometry. (E) Flow cytometric analysis of frequencies of IL-5 + IL-13 + in lung ILC2s after cell stimulation cocktail treatment for 4 h. Papain treated groups were analyzed. (F–J) WT and ICAM-1 −/− mice were intranasally challenged with extract of A. alternata for 4 d ( n = 7). Mice were sacrificed 24 h after the last challenge. (F) The number of eosinophils in BAL was evaluated by flow cytometry. (G) The amount of IL-5 and IL-13 in BAL was measured by ELISA. (H) Total number of ILC2 in lung after A. alternata treatment. (I) The frequencies of IL-5 + IL-13 + in lung ILC2s after cell stimulation cocktail treatment for 4 h. (J) Representative H&E staining of lung sections in A. alternata –treated groups (bars, 100 µm). Data are representative of two independent experiments. Error bars show mean ± SEM; **, P < 0.01; ***, P < 0.001 by unpaired Student's t test. Numbers within flow plots indicate the percentages of cells gated.

Journal: The Journal of Experimental Medicine

Article Title: ICAM-1 controls development and function of ILC2

doi: 10.1084/jem.20172359

Figure Lengend Snippet: ICAM-1 deficiency attenuates papain and A. alternata –induced lung inflammation. (A–E) WT and ICAM-1 −/− mice were intranasally administered with papain or PBS for 5 d ( n = 6 for each group). Mice were sacrificed 24 h after the last treatment. (A) The frequencies and number of eosinophils in BAL were evaluated by flow cytometry. (B) The amount of IL-5 and IL-13 in BAL was measured by ELISA. (C) Representative lung histology by H&E staining (bars, 100 µm). (D) The frequencies and number of lung ILC2s were determined by flow cytometry. (E) Flow cytometric analysis of frequencies of IL-5 + IL-13 + in lung ILC2s after cell stimulation cocktail treatment for 4 h. Papain treated groups were analyzed. (F–J) WT and ICAM-1 −/− mice were intranasally challenged with extract of A. alternata for 4 d ( n = 7). Mice were sacrificed 24 h after the last challenge. (F) The number of eosinophils in BAL was evaluated by flow cytometry. (G) The amount of IL-5 and IL-13 in BAL was measured by ELISA. (H) Total number of ILC2 in lung after A. alternata treatment. (I) The frequencies of IL-5 + IL-13 + in lung ILC2s after cell stimulation cocktail treatment for 4 h. (J) Representative H&E staining of lung sections in A. alternata –treated groups (bars, 100 µm). Data are representative of two independent experiments. Error bars show mean ± SEM; **, P < 0.01; ***, P < 0.001 by unpaired Student's t test. Numbers within flow plots indicate the percentages of cells gated.

Article Snippet: For A. alternata –induced lung inflammation, mice were intranasally administrated with 100 µg A. alternata (Greerlabs) in 40 µl PBS for four consecutive days as previously described ( ).

Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay, Staining, Cell Stimulation

Comparison of glucoamylase production and specific activity by using various raw media for growth of A. alternata.

Journal: BioMed Research International

Article Title: Production of Glucoamylase from Novel Strain of Alternaria Alternata under Solid State Fermentation

doi: 10.1155/2022/2943790

Figure Lengend Snippet: Comparison of glucoamylase production and specific activity by using various raw media for growth of A. alternata.

Article Snippet: A. alternata (MBL-T) is the vital and cheapest microbial source of glucoamylase.

Techniques: Comparison, Activity Assay

Study of glucoamylase production and its specific activity on different weight of raw media by growth of A. alternata.

Journal: BioMed Research International

Article Title: Production of Glucoamylase from Novel Strain of Alternaria Alternata under Solid State Fermentation

doi: 10.1155/2022/2943790

Figure Lengend Snippet: Study of glucoamylase production and its specific activity on different weight of raw media by growth of A. alternata.

Article Snippet: A. alternata (MBL-T) is the vital and cheapest microbial source of glucoamylase.

Techniques: Activity Assay

Comparison of glucoamylase production and specific activity by using various moisture contents for growth of A. alternata.

Journal: BioMed Research International

Article Title: Production of Glucoamylase from Novel Strain of Alternaria Alternata under Solid State Fermentation

doi: 10.1155/2022/2943790

Figure Lengend Snippet: Comparison of glucoamylase production and specific activity by using various moisture contents for growth of A. alternata.

Article Snippet: A. alternata (MBL-T) is the vital and cheapest microbial source of glucoamylase.

Techniques: Comparison, Activity Assay

Optimization of glucoamylase production and specific activity by using different concentration of starch by A. alternata.

Journal: BioMed Research International

Article Title: Production of Glucoamylase from Novel Strain of Alternaria Alternata under Solid State Fermentation

doi: 10.1155/2022/2943790

Figure Lengend Snippet: Optimization of glucoamylase production and specific activity by using different concentration of starch by A. alternata.

Article Snippet: A. alternata (MBL-T) is the vital and cheapest microbial source of glucoamylase.

Techniques: Activity Assay, Concentration Assay, Starch

Use of different nitrogen additives to study the glucoamylase production and specific activity under solid state fermentation by A. alternata.

Journal: BioMed Research International

Article Title: Production of Glucoamylase from Novel Strain of Alternaria Alternata under Solid State Fermentation

doi: 10.1155/2022/2943790

Figure Lengend Snippet: Use of different nitrogen additives to study the glucoamylase production and specific activity under solid state fermentation by A. alternata.

Article Snippet: A. alternata (MBL-T) is the vital and cheapest microbial source of glucoamylase.

Techniques: Activity Assay

Use of different nitrogen additives to study the glucoamylase production and specific activity under solid state fermentation by A. alternata.

Journal: BioMed Research International

Article Title: Production of Glucoamylase from Novel Strain of Alternaria Alternata under Solid State Fermentation

doi: 10.1155/2022/2943790

Figure Lengend Snippet: Use of different nitrogen additives to study the glucoamylase production and specific activity under solid state fermentation by A. alternata.

Article Snippet: A. alternata (MBL-T) is the vital and cheapest microbial source of glucoamylase.

Techniques: Activity Assay

Principal component analysis (PCA) and loading plot-derived correlation between different ecocultural conditions for glucoamylase production by using A. alternata through solid substrate fermentation and partial least squares-discriminant analysis (PLS-DA) score plot indicated the variation between different treatments of ecocultural parameters to optimize the best treatment among all.

Journal: BioMed Research International

Article Title: Production of Glucoamylase from Novel Strain of Alternaria Alternata under Solid State Fermentation

doi: 10.1155/2022/2943790

Figure Lengend Snippet: Principal component analysis (PCA) and loading plot-derived correlation between different ecocultural conditions for glucoamylase production by using A. alternata through solid substrate fermentation and partial least squares-discriminant analysis (PLS-DA) score plot indicated the variation between different treatments of ecocultural parameters to optimize the best treatment among all.

Article Snippet: A. alternata (MBL-T) is the vital and cheapest microbial source of glucoamylase.

Techniques: Derivative Assay

Principal component analysis (PCA) and loading plot-derived correlation between different ecocultural conditions to determine specific activity by using A. alternata through solid substrate fermentation and partial least squares-discriminant analysis (PLS-DA) score plot indicated the variation between different treatments of ecocultural parameters to optimize the best treatment among all.

Journal: BioMed Research International

Article Title: Production of Glucoamylase from Novel Strain of Alternaria Alternata under Solid State Fermentation

doi: 10.1155/2022/2943790

Figure Lengend Snippet: Principal component analysis (PCA) and loading plot-derived correlation between different ecocultural conditions to determine specific activity by using A. alternata through solid substrate fermentation and partial least squares-discriminant analysis (PLS-DA) score plot indicated the variation between different treatments of ecocultural parameters to optimize the best treatment among all.

Article Snippet: A. alternata (MBL-T) is the vital and cheapest microbial source of glucoamylase.

Techniques: Derivative Assay, Activity Assay